Microbial cell factories
Authors:
Fatemeh Ashrafan, Shahrbanoo Keshavarz Azizi Raftar, Arezou Lari, Arefeh Shahryari, Sara Abdollahiyan, Hamid Reza Moradi, Morteza Masoumi, Mehdi Davari, Shohreh khatami, Mir Davood Omrani, Farzam Vaziri, Andrea Masotti and Seyed Davar Siadat
Abstract
Background: Several studies have shown that probiotics have benefcial efects on weight control and metabolic
health. In addition to probiotics, recent studies have investigated the efects of paraprobiotics and postbiotics. Therefore, we evaluated the preventive efects of live and pasteurized Akkermansia muciniphila MucT (A. muciniphila) and its
extracellular vesicles (EVs) on HFD-induced obesity.
Results: The results showed that body weight, metabolic tissues weight, food consumption, and plasma metabolic
parameters were increased in the HFD group, whereas A. muciniphila preventive treatments inhibited these HFD.
The efects of pasteurized A. muciniphila and its extracellular vesicles were more noticeable than its active form. The
HFD led to an increase in the colonic, adipose tissue, and liver infammations and increased the expression of genes
involved in lipid metabolism and homeostasis. Nevertheless, these efects were inhibited in mice that were administered A. muciniphila and its EVs. The assessment of the gut microbiota revealed signifcant diferences in the microbiota composition after feeding with HFD. However, all treatments restored the alterations in some bacterial genera and closely resemble the control group. Also, the correlation analysis indicated that some gut microbiota might be
associated with obesity-related indices.
Conclusions: Pasteurized A. muciniphila and its EVs, as paraprobiotic and postbiotic agents, were found to play a key
role in the regulation of metabolic functions to prevent obesity, probably by afecting the gut-adipose-liver axis.